HT22 mSirt2 S368D Mutant Cell Line

Description

HT22 mSirt2_p.S368D_c.1102T>G_c.1103C>A cell line

Parental cell line: HT22

Species: Mouse

Gene ID: 64383

Mutation: p.S368D_c.1102T>G_c.1103C>A

Genotype: Homozygote

Doubling time: 24-36 h

Growth properties: Adherent

Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media:
DMEM + 30% FBS + 10% DMSO.