Description
BXPC-3 hKRAS_p.G12D_c.35G>A cell line
Parental cell line: BXPC-3
Species: Human
Gene ID: 3845
Mutation: p.G12D_c.35G>A
Genotype: Homozygote
Doubling time: 36-48h
Growth properties: Adherent
Recommended culture media:
RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.
Freezing media:
RPMI-1640 + 30% FBS + 10% DMSO.
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