B16-F10#Itgb3 mSrc E99A Mutant Cell Line

Description

B16-F10#Itgb3 mSrc_p.E99A_c.296A>C cell line

Parental cell line: B16-F10#Itgb3

Species: mouse

Gene ID: 20779

Mutation: p.E99A_c.296A>C

Genotype: Homozygote

Doubling time: 18-24h

Growth properties: Adherent

Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media:
DMEM + 30% FBS + 10% DMSO.