SW48 hNRAS Q61H Mutant Cell Line

Description

SW48 hNRAS_p.Q61H_c.183A>C cell line

Parental cell line: SW48

Species: Homo sapiens, human

Gene ID: 4893

Mutation: p.Q61H_c.183A>C

Genotype: Homozygote

Doubling time: 60-72h

Growth properties: Adherent

Recommended culture media:
Leibovitz L-15 Medium supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 0% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media:
L-15+ 30% FBS + 10% DMSO.