hCMEC/D3 hKSR2 rs12822146 mutant cell line

Description

hCMEC/D3 hKSR2_rs12822146 cell line

Parental cell line: hCMEC/D3

Species: Homo sapiens, human

Gene ID: 283455

Mutation: rs12822146

Genotype: Homozygote

Doubling time: 36-54h

Growth properties: Adherent

Recommended culture media:
Immortalized Human Brain Microvascular Endothelial Cell Basal Medium supplemented with 5% FBS, 1 % Endothelial Cell Growth Supplement, and 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media:
60% basal medium + 30% FBS + 10% DMSO.