Description
MEG-01 hJAK2_p.V617F_c.1849G>T cell line
Parental cell line: MEG-01
Species: Human
Gene ID: 3717
Mutation: p.V617F_c.1849G>T
Genotype: Homozygote
Doubling time: 36-48h
Growth properties: Semi-adherent
Recommended culture media:
RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells grow in an adherent-suspension mixed state at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.
Freezing media:
RPMI-1640 + 30% FBS + 10% DMSO.
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