Description
hCMEC/D3 hRNF213_p.R4810K_c.14429G>A cell line
Parental cell line: hCMEC/D3
Species: Human
Gene ID: 57674
Mutation: p.R4810K_c.14429G>A
Genotype: Homozygote
Doubling time: 24-36h
Growth properties: Adherent
Recommended culture media:
RPMI-1640 supplemented with 20 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 5×10^5 to 1×10^6 cells per mL.
Freezing media:
RPMI-1640 + 30% FBS + 10% DMSO.
Reviews
There are no reviews yet.