Immortalized Human Primary Renal Glomerular Endothelial Cells

Description

Glomerular endothelial cells (GECs) are specialized microvascular cells involved in regulating glomerular ultrafiltration. They form the inner part of the filtration barrier and participate in the pathophysiological processes of the glomerulus. GECs continuously produce bioactive molecules that can be amplified by inflammatory and thrombotic factors. Endothelial injury caused by severe glomerular lesions inhibits angiogenesis and leads to sclerosis at the injured site. GEC injury affects mesangial cells and epithelial cells, resulting in the deterioration of nephropathy. Due to the great difficulties in the culture, cloning and propagation of GECs, their biological characteristics remain largely unknown.

Immortalized Human Renal Glomerular Endothelial Cells were transduced with lentivirus carrying the SV40 gene and puromycin resistance. Tests confirmed that the cells are free from bacterial and fungal contamination, exhibit excellent proliferation potential, and can be passaged 10?20 times in vitro.