ARPE-19 hGUCY2D R838S Mutant Cell Line

Description

ARPE-19 hGUCY2D_p.R838S_c.2512C>A cell line

Parental cell line: ARPE-19

Species: Homo sapiens, human

Gene ID: 3000

Mutation: p.R838S_c.2512C>A

Genotype: Homozygote

Doubling time: 36-48h

Growth properties: Adherent

Recommended culture media:
Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 µg/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media:
D/F12 + 30% FBS + 10% DMSO.