HepG2 PIK3R1 T603G Mutant Cell Line

Size: 1×10^6 cells/ vial

For research use only

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Description

HepG2 PIK3R1_p.T603G_c.1807A>G_c.1808C>G_c.1809 T>A cell line

Parental cell line: Hep-G2

Species: Homo sapiens, human

Gene ID: 5295

Mutation: PIK3R1_p.T603G_c.1807A>G_c.1808C>G_c.1809 T>A

Genotype: Homozygote, Hetrozygote

Doubling time: 72-96h

Growth properties: Adherent

Recommended culture media:
DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media:
60%DMEM-H + 30% FBS + 10% DMSO.

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