Description
HepG2 PIK3R1_p.T603G_c.1807A>G_c.1808C>G_c.1809 T>A cell line
Parental cell line: Hep-G2
Species: Homo sapiens, human
Gene ID: 5295
Mutation: PIK3R1_p.T603G_c.1807A>G_c.1808C>G_c.1809 T>A
Genotype: Homozygote, Hetrozygote
Doubling time: 72-96h
Growth properties: Adherent
Recommended culture media:
DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.
Freezing media:
60%DMEM-H + 30% FBS + 10% DMSO.







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