Description
THP-1 hLACC1_p.V254I_c.760G>A cell line
Parental cell line: THP-1
Species: Homo sapiens, human
Gene ID: 144811
Mutation: p.V254I_c.760G>A
Genotype: Homozygote
Doubling time: 24-36h
Growth properties: Suspension
Recommended culture media:
RPMI-1640 supplemented with 10 % FBS, 0.05 mM 2-mercaptoethanol, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured in suspension at 37 °C in a humidified atmosphere with 5% CO2 and passaged every 2-3 days at a density of 2-3×10^6 cells/mL, with a seeding density of 5×10^5 to 1×10^6 cells/mL.
Freezing media:
RPMI-1640 + 30% FBS + 10% DMSO.
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