Description
Jurkat hLCK_p.C476A_c.1426T>G_c.1427G>C cell line
Parental cell line: Jurkat
Species: Human
Gene ID: 3932
Mutation: p.C476A_c.1426T>G_c.1427G>C
Genotype: Homozygote
Doubling time: 36-48h
Growth properties: Suspension
Recommended culture media:
RPMI-1640 supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured in suspension at 37 °C in a humidified atmosphere with 5% CO2 and passaged every 2-3 days at a density of 2-3×10^6 cells/mL, with a seeding density of 5×10^5 to 1×10^6 cells/mL.
Freezing media:
RPMI-1640 + 30% FBS + 10% DMSO.
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