Description
SK-MEL-1 hBRD4_c.1213-1G>T cell line
Parental cell line: SK-MEL-1
Species: Human
Gene ID: 23476
Mutation: c.1213-1G>T
Genotype: Homozygote
Doubling time: 48-60h
Growth properties: Suspension
Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured in suspension at 37 °C in a humidified atmosphere with 5% CO2 and passaged every 2-3 days at a density of 2-3×10^6 cells/mL, with a seeding density of 5×10^5 to 1×10^6 cells/mL.
Freezing media:
DMEM + 30% FBS + 10% DMSO.
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