Description
Hep-G2 hUBE2O_p.K689R_c.2066A>G cell line
Parental cell line: Hep-G2
Species: Human
Gene ID: 63893
Mutation: p.K689R_c.2066A>G
Genotype: Homozygote
Doubling time: 60-72h
Growth properties: Adherent
Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.
Freezing media:
DMEM + 30% FBS + 10% DMSO.
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