Description
A-549 hRB1CC1_p.R474H_c.1421G>A_c.1422C>T cell line
Parental cell line: A-549
Species: Human
Gene ID: 9821
Mutation: p.R474H_c.1421G>A_c.1422C>T
Genotype: Homozygote
Doubling time: 36-48h
Growth properties: Adherent
Recommended culture media:
Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.
Freezing media:
D/F12 + 30% FBS + 10% DMSO.
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