Description
MPX NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two NOMAD Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPX NOMAD CCK2 Cholecystokinin Receptor Cell Line contains U2OS cells stably expressing the following constructs:
- human CCK2 Cholecystokinin Receptor with no tag
- Calcium Nomad Biosensor (Green Fluorescence)
- β-arrestin Nomad Biosensor (Red Fluorescence)
MPX NOMAD CCK2 Cholecystokinin Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to CCK2R a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
