Description
MPX NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPX NOMAD CB1 Cannabinoid Receptor Cell Line contains HEK293 cells stably expressing the following constructs:
- human α1B-adrenoceptor with no tag
- cAMP Nomad Biosensor (Red Fluorescence)
- β-arrestin Nomad Biosensor (Green Fluorescence)
MPX NOMAD CB1 Cannabinoid Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by cAMP flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to CB1 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and cAMP biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
