Description
U2OS Cell Line stably expressing D2 Dopamine Receptor & cAMP Biosensor has developed using NOMAD technology. Each vial of these cells stably expressing the following constructs:
- human D2 Dopamine Receptor (DRD2) with no tag;
- cAMP NOMAD Biosensor (Red Fluorescence).
The U2OS Cell Line stably expressing D2 Dopamine Receptor & cAMP Biosensor allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to DRD2, the Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
