Description
NCI-H446?hHFE_p.C282Y_c.845G>A cell line
Parental cell line: NCI-H446
Species: Human
Gene ID: 3077
Mutation: p.C282Y_c.845G>A
Genotype: Homozygote
Doubling time: 48-72h
Growth properties: Semi-adherent
Recommended culture media:
RPMI-1640 supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells grow in an adherent-suspension mixed state at 37 °C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.
Freezing media:
RPMI-1640 + 30% FBS + 10% DMSO.
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