Description
PARP14 point mutant HeLa cell line
Parental cell line: HeLa
Species: Homo sapiens, human
Gene ID:?54625
Mutation: PARP14_p.H1682Q_c.5046T>G
Genotype: Homozygote
Doubling time: 30-48h
Growth properties: Adherent
Recommended culture media:
Minimum Essential Medium (MEM) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 µg/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:6.
Freezing media:
MEM + 30% FBS + 10% DMSO.
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