HEK293 cells stably expressing S1P3 Lysophospholipid Receptor, cAMP biosensor & ß-arrestin biosensor

Size: 2 vials, 3 x 10^6 cells/ vial

For research use only

Request A Quote

Description

NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two Nomad Biosensors to monitor G-protein and β-arrestin modulation. Each vial of NOMAD S1P3 Lysophospholipid Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

  • human S1P3 Lysophospholipid Receptor with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)
  • β-arrestin Nomad Biosensor (Green Fluorescence)

NOMAD S1P3 Lysophospholipid Receptor Cell Line allows to assay compounds analyzing both signaling pathways involving receptor activation; G protein by cAMP flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to S1P3R a G protein is activated which, in turn, triggers a cellular response mediated by cAMP and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and cAMP biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Data Sheet

Download

Cell Culture Manual

Download