Description
cAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP NOMAD Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMP NOMAD D1 Dopamine Receptor (DRD1) Cell Line contains HEK293 cells stably expressing the following constructs:
- human D1 Dopamine Receptor (DRD1) with no tag;
- cAMP NOMAD Biosensor (Red Fluorescence).
cAMP NOMAD D1 Dopamine Receptor (DRD1) Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to DRD1, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
