Description
HCT116 hCMPK1_p.G8R_c.22G>C cell line
Parental cell line: HCT116
Species: Homo sapiens, human
Gene ID: 51727
Mutation: p.G8R_c.22G>C
Genotype: Homozygote
Doubling time: 24-36h
Growth properties: Adherent
Recommended culture media:
McCoy’s 5A Medium supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:8.
Freezing media:
McCoy’s 5A + 30% FBS + 10% DMSO.
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