Description
cAMP NOMAD ADRA2B Receptor Cell Line
cAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMP NOMAD ADRA2B Receptor Cell Line contains U2OS cells stably expressing the following constructs:
- Human α2B-adrenoceptor with no tag
- cAMP Nomad Biosensor (Green Fluorescence)
cAMP NOMAD ADRA2B Receptor Cell Line allows to assay compounds analyzing the G-Protein signaling pathway by cAMP involving receptor activation. When an agonist binds to ADRA2B, a Gi protein is activated, thus inhibiting adenylate cyclase and typically decreasing intracellular cAMP levels. However, the process in this cAMP NOMAD ADRA2B Cell Line results in an increase in the green fluorescence intensity of the cAMP biosensor, which is engineered to respond in this way. Thus, receptor activity can be easily quantified in living cells in a dose-dependent manner by measuring fluorescence emission with a microplate reader.
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