Description
Chrne1 point mutant C2C12 cell line
Parental cell line: C2C12
Species: mouse
Gene ID: 11435
Mutation: Chrna1_p.F253V_c.757T>G
Genotype: Homozygote
Doubling time: 24-36h
Growth properties: Adherent
Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate, 100 U/mL Penicillin and 100 µg/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.
Freezing media:
DMEM + 30% FBS + 10% DMSO.
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