U-251MG hFOXG1 Q86fs*35 Mutant Cell Line

Description

U-251MG hFOXG1_c.256dupC cell line

Parental cell line: U-251MG

Species: Homo sapiens, human

Gene ID: 2290

Mutation: FOXG1_p.Q86fs*35_c.256dupC

Genotype: Homozygote

Doubling time: 24-36h

Growth properties: Adherent

Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 µg/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media:
DMEM + 30% FBS + 10% DMSO.