HeLa PARP14 H1682Q Mutant Cell Line

Description

PARP14 point mutant HeLa cell line

Parental cell line: HeLa

Species: Homo sapiens, human

Gene ID:?54625

Mutation: PARP14_p.H1682Q_c.5046T>G

Genotype: Homozygote

Doubling time: 30-48h

Growth properties: Adherent

Recommended culture media:
Minimum Essential Medium (MEM) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 µg/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:6.

Freezing media:
MEM + 30% FBS + 10% DMSO.