Description
TMD8 hBTK_p.L528W_c.1583T>G cell line
Parental cell line: TMD8
Species: Human
Gene ID: 695
Mutation: p.L528W_c.1583T>G
Genotype: Homozygote
Doubling time: 36-48h
Growth properties: Adherent
Recommended culture media:
RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 5?10? to 1?10? cells per mL.
Freezing media:
RPMI-1640 + 30% FBS + 10% DMSO.
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