PC12 rFgfr1 N546K Mutant Cell Line

Description

PC12 rFgfr1_p.N546K_c.1638C>TG cell line

Parental cell line: PC12

Species: Rat

Gene ID: 79114

Mutation: p.N546K_c.1638C>TG

Genotype: Homozygote

Doubling time: 36-48h

Growth properties: Semi-adherent

Recommended culture media:
RPMI-1640 supplemented with 5 % FBS, 10 % HS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells grow in an adherent-suspension mixed state at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media:
RPMI-1640 + 30% FBS + 10% DMSO.