Description
HEK293T hNUP107_p.R454W_c.1360C>T cell line
Parental cell line: HEK293T
Species: Human
Gene ID: 57122
Mutation: p.R454W_c.1360C>T
Genotype: Homozygote
Doubling time: 24-48 h
Growth properties: Adherent
Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.
Freezing media:
DMEM + 30% FBS + 10% DMSO.
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