Description
MCF-7 hAKT1_p.E17K_c.571G>A cell line
Parental cell line: MCF-7
Species: Human
Gene ID: 207
Mutation: p.E17K_c.571G>A
Genotype: Homozygote
Doubling time: 36-48h
Growth properties: Adherent
Recommended culture media:
Minimum Essential Medium (MEM) supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate,1 %NEAA, 10 ug/mL Human recombinant insulin, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.
Freezing media:
MEM + 30% FBS + 10% DMSO.
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