AC16 hRYR2 D179E Mutant Cell Line

Description

AC16 hRYR2_p.D179E_c.537T>G cell line

Parental cell line: AC16

Species: Rat

Gene ID: 6262

Mutation: p.D179E_c.537T>G

Genotype: Homozygote

Doubling time: 24-36h

Growth properties: Adherent

Recommended culture media:
Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 12.5 % FBS, 2 mM Glutamax, 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media:
D/F12 + 30% FBS + 10% DMSO.