Description
CaNOMAD Muscarinic Acetylcholine Receptor M1 (CHRM1) Cell Line
CaNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and Calcium Nomad Biosensor. These cells can monitor G-protein modulation by intracellular calcium release. Each vial of CaNOMAD CHRM1 Receptor Cell Line contains U2OS cells stably expressing the following constructs:
- Human Muscarinic Receptor 1 (CHRM1) with no tag
- Calcium Nomad Biosensor (Green Fluorescence)
CaNOMAD Muscarinic Acetylcholine Receptor M1 (CHRM1) Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gq/11 involving receptor activation.
When an agonist binds to M1 receptor, the Gq/G11 protein is activated which, in turn, triggers a cellular response mediated by calcium via Phospholipase C stimulation. This leads to an increase of green fluorescence intensity of Calcium biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
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