Description
Muscarinic Acetylcholine Receptor M4 Cell Line
icAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and PKA Nomad Biosensor to monitor G-protein modulation by icAMP activation. Each vial of icAMP NOMAD Muscarinic M4 Receptor Cell Line contains HEK 293 / U2OS cells stably expressing the following constructs:
- Human Muscarinic M4 Receptor (M4R) with no tag
- icAMP Nomad Biosensor (Red Fluorescence)
icAMP NOMAD Muscarinic M4 Receptor Cell Line allows to assay compounds analyzing the G-Protein signaling pathway by Gi/Go involving receptor activation. When an agonist binds to M4 Receptor, the Gi/Go protein is activated which, in turn, triggers a cellular response mediated by icAMP. This leads to an increase of red fluorescence intensity of icAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
Reviews
There are no reviews yet.