Hep-G2 hPCF11 S590R Mutant Cell Line

Description

Hep-G2 hPCF11_p.S590R_c.1770T>A cell line

Parental cell line: Hep-G2

Species: Human

Gene ID: 51585

Mutation: p.S590R_c.1770T>A

Genotype: Homozygote

Doubling time: 60-72h

Growth properties: Adherent

Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate, 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media:
DMEM + 30% FBS + 10% DMSO.