Description
cAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMP NOMAD Adenosine A3 Receptor Cell Line contains HEK293 cells stably expressing the following constructs:
- human Adenosine A3 receptor (ADORA3) with no tag;
- cAMP Nomad Biosensor (Red Fluorescence).
cAMP NOMAD ADORA3 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to ADORA3, Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
