HEK293 cell line stably expressing Adenosine A1 Receptor and cAMP Biosensor

Size: 2 vials, 3 x 10^6 cells/ vial

For research use only

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Description

cAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMP NOMAD Adenosine A1 Receptor Cell Line contains HEK293 cells stably expressing the following constructs:

  1. human Adenosine A1 receptor (ADORA1) with no tag;
  2. cAMP Nomad Biosensor (Red Fluorescence).

cAMP NOMAD ADORA1 Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Go involving receptor activation. When an agonist binds to ADORA1, Go protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

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