Description
Green Fluorescent NK3 Tachykinin Receptor Cell Line contains SH-SY5Y cells stably expressing NK3 Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual. The Green Fluorescent NK3 Tachykinin Receptor Cell Line allows to assay compounds, or analyze their capability to modulate NK3 Receptor activation and the following redistribution process inside the cells. When a ligand binds to the NK3 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent NK3 Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring NK3 Receptor redistribution process in High Throughput Screening.
