Description
Novel Green Fluorescent Nuclear Localization Signal Cell Lines (U2OS or HEK293) have been developed through stable transfection of turboGFP tagged in the N-terminal with the NLS in U2OS or HEK293 cell lines. Both cell lines allow the study of the translocation processes between the nucleus and the cytoplasm. Nuclear localization signals (NLS) are short peptide sequences within the amino acid sequence of nuclear cargo proteins. Commonly, the NLS sequences contain a high proportion of lysine (K) or arginine (R) aminoacids and are very similar to the first found NLS sequence in the simian virus SV40 (PKKKRKV).
Each vial of both U2OS/NLS-tGFP and HEK293/NLS-tGFP cell lines contains 3 million cells. It is guaranteed that these cells will expand following the cell culture manual.
