Description
Green Fluorescent PAC1 Receptor Cell Line contains U2OS cells stably expressing PAC1 Receptor tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual. The Green Fluorescent PAC1 Receptor Cell Line allows to assay compounds, or analyze their capability to modulate PAC1 Receptor activation and the following redistribution process inside the cells. When a ligand binds to the PAC1 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent PAC1 Receptor redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring PAC1 Receptor redistribution process in High Throughput Screening.