Description
A novel HEK293 Cell line, Fluorescent Glucocorticoid Receptor Nuclear Translocation Assay Cell Line, has been developed through stable transfection of an expression vector for a fusion protein of turboGFP and human Glucocorticoid Receptor (h-GR). The resultant cell line allows to monitor the dynamics of the hormone/receptor complex in living cells and to assay for compounds inducing tGFP-hGR nuclear translocation and formation of nuclear foci.
The Fluorescent Glucocorticoid Receptor Nuclear Translocation Assay Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate glucocorticoid receptor nuclear translocation process and quantifying the fluorescence distribution inside the cells. This highly reproducible assay allows monitoring glucocorticoid receptor nuclear translocation process in High Content Analysis and fluorescence microscope applications. It is guaranteed that these cells will expand in more than 30 passages following the cell culture manual.
