cAMP NOMAD D4 Dopamine Receptor Cell Line

2 vials, 3×10^6 cells/ vial

For research use only

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Description

cAMP NOMAD D4 Dopamine Receptor Cell Line (DRD4 Cell Line)

cAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by Adenylyl cyclase inhibition or stimulation. Each vial of cAMP NOMAD D4 Dopamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • Human D4 Dopamine Receptor (DRD4) with no tag
  • cAMP Nomad Biosensor (Green Fluorescence)

cAMP NOMAD DRD4 Cell Line allows to assay compounds analyzing the G-Protein signaling pathway by Go involving receptor activation. When an agonist binds to DRD4, the Go protein turns activated which, in turn, triggers a cellular response mediated by cAMP (Adenylyl cyclase inhibition).

This leads to an increase of green fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

DRD4 encodes the D4 subtype of the dopamine receptor. The D4 subtype inhibits adenylyl cyclase through inhibitory G-proteins. Dopamine Receptor D4 (DRD4) is a gene that codes for a receptor protein involved in neurotransmission, located on chromosome 11p15.5. It has common polymorphic variants in the human population, with a specific deletion leading to autonomic hyperactivity in some individuals.

DRD4 is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes. These include autonomic nervous system dysfunction, attention deficit/ hyperactivity disorder, and the personality trait of novelty seeking. cAMP NOMAD D4 Dopamine Receptor Cell Line allows the measurement of the activity of ligands, such as Dopamine.

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Data Sheet

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Assay Protocol

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