cAMP NOMAD D3 Dopamine Receptor Cell Line

2 vials, 3×10^6 cells/ vial

For research use only

Description

cAMP NOMAD D3 Dopamine Receptor Cell Line (DRD3 Cell Line)

cAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by Adenylyl cyclase inhibition or stimulation. Each vial of cAMP NOMAD D3 Dopamine Receptor Cell Line contains U2OS cells stably expressing the following constructs:

Human D3 Dopamine Receptor (DRD3) with no tag
cAMP Nomad Biosensor (Green Fluorescence)

cAMP NOMAD DRD3 Cell Line allows to assay compounds analyzing the G-Protein signaling pathway by Go involving receptor activation. When an agonist binds to DRD3, the Go protein is activated which, in turn, triggers a cellular response mediated by cAMP (Adenylyl cyclase inhibition).

This leads to an increase of green fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.