ApoE4 Expressing HEK293 Cell Line

Description

We provide the following three APOE4 stable cell lines. These cell lines are powerful tools for studying the molecular and cellular mechanisms underlying neurodegenerative diseases, including Alzheimer’s disease.

• ApoE4 Stably Expressing Immortalized Human Astrocytes (P30758)
• ApoE4 Stably Expressing Immortalized Human Microglia (P30759)
• ApoE4 Stably Expressing HEK293 Cell Line (P30757)

HEK293 overexpressing ApoE4-FLAG are stably engineered to overexpress the ApoE4 isoform tagged with a C-terminal FLAG epitope, enabling easy detection and purification. Protein overexpression has been validated by both immunofluorescence and Western blot. Additionally, active secretion of ApoE4 into the culture medium has been confirmed via ELISA, ensuring functional expression and suitability for studies on lipid metabolism, neuroinflammation, and neurodegenerative disease mechanisms. Developed with precision and reproducibility in mind, our APOE4-expressing cell lines are optimized for a wide range of applications, from in vitro disease modeling to high-throughput screening.