Description
Amyloid precursor protein (APP) Processing Assay Cell Line is an in vitro Alzheimer’s Disease model that can identify Aβ peptide formation inhibitors such as secretase activity inhibitors. It consists in MDCK cell line stably expressing green fluorescent human amyloid precursor protein (APP). To perform the assay, cells are incubated with test compounds for 48-72 hours to evaluate their effects on APP Processing. After incubation, nucleus could be stained with DAPI and retained fluorescent APP vesicles could be detected by fluorescence using image analysis algorithms. It is guaranteed that these cells can expand in more than 30 passages following the cell culture manual.