THP-1 hTLR8 V434L Mutant Cell Line

Description

THP-1 hTLR8_p.V434L_c.1300G>C cell line

Parental cell line: THP-1

Species: Homo sapiens, human

Gene ID: 51311

Mutation: p.V434L_c.1300G>C

Genotype: Homozygote

Doubling time: 24-36h

Growth properties: Suspension

Recommended culture media:
RPMI-1640 supplemented with 10 % FBS, 0.05 mM 2-mercaptoethanol, 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells are cultured in suspension at 37 °C in a humidified atmosphere with 5% CO2 and passaged every 2-3 days at a density of 2-3×10^6 cells/mL, with a seeding density of 5×10^5 to 1×10^6 cells/mL.

Freezing media:
RPMI-1640 + 30% FBS + 10% DMSO.