Huh-7 hACSM5 P352R Mutant Cell Line

Description

Huh-7 hACSM5_p.P352R_c.1055C>G cell line

Parental cell line: Huh-7

Species: Human

Gene ID: 54988

Mutation: p.P352R_c.1055C>G

Genotype: Homozygote

Doubling time: 36-48h

Growth properties: Adherent

Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 2 mM Glutamax, 1 mM Sodium Pyruvate, 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media:
DMEM + 30% FBS + 10% DMSO.