Description
HCT 116 hMAP2K1_p.P124S_c.371C>T cell line
Parental cell line: HCT 116
Species: Human
Gene ID: 5604
Mutation: p.P124S_c.371C>T
Genotype: Heterozygote
Doubling time: 24-36h
Growth properties: Adherent
Recommended culture media:
McCoy’s 5A Medium supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.
Subculture:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:8.
Freezing media:
McCoy’s 5A + 30% FBS + 10% DMSO.
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