LL/2 (LLC1) mEgfr L860R Mutant Cell Line

Description

LL/2 (LLC1) mEgfr_p.L860R_c.2579T>G cell line

Parental cell line: LL/2 (LLC1)

Species: Mouse

Gene ID: 13649

Mutation: p.L860R_c.2579T>G

Genotype: Homozygote

Doubling time: 18-24h

Growth properties: Semi-adherent

Recommended culture media:
Dulbecco’s Modified Eagle Medium, High Glucose (DMEM) supplemented with 10 % FBS, 100 U/mL Penicillin and 100 ug/mL Streptomycin.

Subculture:
Cells grow in an adherent-suspension mixed state at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media:
DMEM + 30% FBS + 10% DMSO.